Hydrolytic Enzymes of Rabbit Mononuclear Exudate Cells

Hydrolytic Enzymes of Rabbit Mononuclear Exudate Cells. Ii. Lysozyme: Properties and Quantitative Assay in Tuberculous and Control Inbred Rabbits.

HYDROLYTIC ENZYMES OF RABBIT MONONUCLEAR EXUDATE CELLS I. Quantitative Assay and Properties of Certain Proteases, Non-Specific Esterases, and Lipases of Mononuclear and Polymorphonuclear Cells and Erythrocytes

Oil-induccd mononuclear phagocytes (MN) were quantitatively assayed for various hydrolases as unfractionatcd suspensions of frozen and thawed cells. They apparently contain two proteases. The first, measured with ureaor acid-denatured hemoglobin, was similar to purified Proteinase I of lung with respect to pH optimum (pH 4), stability, hydrolytic and polymerizing activities, and reactions to va...

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

The cytochrome oxidase (CO), aminopeptidase (AMP), succinic dehydrogenase (SD), acid phosphatase, esterase, and alkaline phosphatase of rabbit mononuclear (MN) and polymorphonuclear (PMN) peritoneal exudate cells and pulmonary alveolar macrophages (AM) - air dried on Mylar strips - were characterized by histochemical techniques with respect to stability, activators, inhibitors, and pH optima. A...

Chemotaxis of Mononuclear Cells

Chemotaxis of rabbit mononuclear cells was studied by the micropore-filter technique. Mononuclear cells obtained from mineral oil-induced peritoneal exudates respond chemotactically to rabbit serum treated with immune complexes or with streptokinase and plasminogen, to soluble factors produced by bacteria, and to lysates obtained from rabbit neutrophils. The first chemotactic factor requires he...

Failure of rabbit neutrophils to secrete endogenous pyrogen when stimulated with staphylococci

Cells obtained from acute peritoneal exudates in rabbits were separated into neutrophil and mononuclear populations by centrifugation on colloidal silica gradients. When these populations were separately incubated in tissue culture medium in the presence of opsonized Staphylococcus epidermidis, endogenous pyrogen was secreted only by the adherent cells of the mononuclear population. Pyrogen pro...